Micropropagation of sour cherry (Prunus cerasusL.) cv.

✍ Scribed by Radosav Cerović; Djurdina Ružić

• Book ID: 104620950
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 302 KB
• Volume: 9
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00044251

✦ Synopsis

Sour cherry cv. ~umadinka (Prunus cerasus L.) is the leading Yugoslav cultivar for production orchards. A method of micropropagation has been developed for the purpose of growing '~umadinka' on its own roots and for rapid multiplication.

Aseptic cultures were initiated from shoot explants 1-2 mm long on Murashige & Skoog medium with (in mg 1 -~) 6-benzylaminopurine (BAP): 1, indole -3-yl butyric acid (IBA): 1 and gibberellic acid (GA3): 0-1.

The best medium for proliferation was MS with (in mg 1-~ ): BAP 0.5, IBA 0.1, GA 3 0.1, but media with (in mgl-1): BAP 0.5, NAA 0.1, GA3 0.1 and BAP 1, NAA 0.1 and GA3 0.1 were also shown to be good. A higher degree of proliferation obtained with some media did not necessarily result in a better quality of plantlets produced.

For rooting the best combination of culture medium was achieved with pretreatment 10 days in MS I/2 with 1 mgl -t IBA, followed by transfer to a hormone-free medium after 5-10 days, resulting in 88% success.

The rooted plants were planted in containers and acclimatized under mist, with over 90% of plants surviving transplantation.