Micropropagation of kiwifruit using non-axenic shoot tips

✍ Scribed by Paul L. Monette

• Book ID: 104617385
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 472 KB
• Volume: 6
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00037760

✦ Synopsis

Kiwifruit (Actinidia chinensis Planch.) shoot tips were subjected to a standard surface sterilization procedure and cultured on a Murashige and Skoog basal medium in the presence of two surviving bacterial contaminants. The fresh weight increase of the cultures and the number of shoots produced were greater in liquid medium than in medium solidified with 0.4 or 0.8% agar. A greater number of shoots was obtained with 125ml than with 50, 250, or 500ml Erlenmeyer flasks. A concentration of 2mg1-1 N*-benzylaminopurine (BAP) gave a greater increase in fresh weight than 6ither 0 or 4mg1-1 .

Shoots cut from proliferating cultures were dipped in 0.05% indolebutyric acid (IBA) and rooted directly in a peat :vermiculite :perlite mix. Over 93% of 907 plantlets produced were successfully acclimatized. The productivity of the method was comparable to that reported for the axenic culture of meristems. The contaminants which survived the initial surface sterilization procedure thus presented no major obstacle to the in vitro propagation of kiwifruit.