A micropropagation system for Annona squamosa L. (Sugar Apple) using hypocotyls of seedlings and nodal cuttings from 3-year-old plants was developed. Shoot proliferation was achieved with Woody Plant Medium supplemented with BA. Silver thiosulphate was added at 0.5 mg 1-1 to control leaf abscission.
Micropropagation of juvenile and adultSorbus domesticaL.
โ Scribed by Isabel Arrillaga; Toni Marzo; Juan Segura
- Book ID
- 104647810
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 793 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0167-6857
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โฆ Synopsis
Successful propagation of seedlings and mature trees of Sorbus dornestica L. has been achieved by in vitro methods. Multiple shoot formation was obtained by placing shoot apices or nodal segments on a modified Schenck and Hildebrandt medium containing benzyladenine. Regenerated shoots were excised and induced to root on media with auxin. In the best treatments 75-85% of shoots from juvenile material rooted. Rooting capacity of shoots from mature explants was lower (30%) and was not improved by dipping the base of shoots in concentration solutions of indolebutyric or naphthaleneacetic acids. Plantlets were ultimately established in soil.
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