The multistep assay of specific catechol-O-methyltransferase (COMT) activity in human erythrocytes was validated. Enzyme preparations from lysed erythrocytes were incubated with a substrate (3,4-dihydroxybenzoic acid) in the presence of Mg2+ and S-adenosylmethionine. The reaction products (vanillic
✦ LIBER ✦
Microplate screening assay to identify inhibitors of human catechol-O-methyltransferase
✍ Scribed by Mika Kurkela; Antti Siiskonen; Moshe Finel; Päivi Tammela; Jyrki Taskinen; Pia Vuorela
- Book ID
- 108047709
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 228 KB
- Volume
- 331
- Category
- Article
- ISSN
- 0003-2697
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