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Micropacked capillary nucleic acid analysis

✍ Scribed by Remcho, Vincent T. ;McNair, Harold M.

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
351 KB
Volume
14
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

Nucleic acid compositional analysis is discussed, followed by a brief review of the current state of the technology applied in which the benefits and limitations of current methodology are enumerated. An alternative method is presented, namely the use of a micropacked capillary ion exchange column to separate nucleoside monophosphates via an anion exchange mechanism. The separated nucleotides are then quantitated by means of post‐column photodiode‐array detection. The use of a photodiode array also enables the verification of peak identity and purity by acquisition of UV spectra at any point in the separation. The technique has applications in the compositional analysis of both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).

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