Micronuclei and developmental abnormalities in 4-day mouse embryos after paternal treatment with acrylamide
✍ Scribed by Nina Titenko-Holland; Thomas Ahlborn; Xiu Lowe; Nong Shang; Martyn T. Smith; Andrew J. Wyrobek
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 336 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
The developmental consequences of paternal expo-tive (MN/) were induced. Nuclei of abnormal emsure to acrylamide (50 mg/kg i.p. for 5 days) were bryos were significantly larger (900 mm 2 vs. 250 assessed in preimplantation embryos. There was a mm 2 ) than controls. In addition, MN of abnormal significant increase in the proportion of morphologi-embryos were larger than those of normal embryos cally abnormal embryos after postmeiotic treatment (21.2 mm 2 vs. 6.5 mm 2 , P õ 0.01). Among control during spermatogenesis (88.7% vs. 14.8% in con-embryos, MN/ were significantly larger than MN0 trol). Abnormal embryos had an average of 1.8 { (P õ 0.05). These findings suggest that the preim-3.5 cells and ú80% had at least one fragmented plantation embryo is a sensitive indicator of paternucleus. In addition, morphologically normal em-nally transmitted effects on early development. Mulbryos were significantly delayed (34.3 { 12.8 cells tiple mechanisms appear to be involved, including per embryo vs. 57.6 { 15.7 in control, P õ 0.001). cytogenetic damage, proliferation arrest/delay, and Acrylamide caused 10-and 20-fold increases in fertilization failure. Future studies are needed to esfrequencies of cells with micronuclei (MN) in mor-tablish how induced cytological defects in preimplanphologically normal and abnormal embryos, re-tation embryos contribute to birth defects and other spectively (41 and 93 MN per 1,000 cells). Both postimplantation abnormalities. Environ. Mol. Mutacentromere-negative (MN0) and centromere-posi-gen.