✦ LIBER ✦

Microheterogeneity of apolipoprotein D as revealed by electroblotting following isoelectric focusing in Immobiline DryPlates

✍ Scribed by Dr. Leif Holmquist

Book ID: 102180410
Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 361 KB
Volume: 13
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150130153

No coin nor oath required. For personal study only.

✦ Synopsis

Dryplates

The microheterogeneity of apolipoprotein D was examined by a procedure involving, in sequences: (i) electrophoresis in an immobilized pH 4-7 gradient in an Immobiline DryPlate-polyacrylamide gel supplemented with Ampholine pH 5-7, (ii) covering of the gel with sodium dodecyl sulfate-containing agarose, (iii) electroblotting onto a polyvinylidene difluoride membrane and (iv) immunological identification. Seven isoforms were obtained with partially purified apolipoprotein D. Using this technique the apparent plvalues at 15°C for the isoforms were 4.57,4.67,4.78,4.83 and5.95,6.06and6.19(SDfO.O5forall).Direct stainingofthe Immobiline DryPlate could not reveal the isoforms of partially purified apolipoprotein D.

📜 SIMILAR VOLUMES

Glycosylation of human abumin in diabete

Glycosylation of human abumin in diabetes mellitus: Extensive microheterogeneity of serum and urinary species as revealed by isoelectric focusing

✍ Dr. Giovanni Candiano; Gian Marco Ghiggeri; Gerolamo Delfino; Carlo Queirolo; El 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 735 KB

The Net Surface Charge of Mammalian Sper

The Net Surface Charge of Mammalian Spermatozoa as Determined by Isoelectric Focusing. Changes Following Sperm Maturation, Ejaculation, Incubation in the Female Tract, and after Enzyme Treatment

✍ H. D. M. Moore 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 648 KB