Microfluidic Methods for Molecular Biology

✍ Scribed by Chang Lu, Scott S. Verbridge (eds.)

• Publisher: Springer International Publishing
• Year: 2016
• Tongue: English
• Leaves: 382
• Edition: 1
• Category: Library

✦ Synopsis

This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry.
This book also:

• Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis
  
• Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes
  
• Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics
  

✦ Table of Contents

Front Matter....Pages i-xii
Microfluidic Platforms for Quantitative Biology Studies in Model Organisms....Pages 1-18
Microfluidic Methods in Single Cell Biology....Pages 19-54
Convective PCR Thermocycling with Smartphone-Based Detection: A Versatile Platform for Rapid, Inexpensive, and Robust Mobile Diagnostics....Pages 55-69
Forensic Typing of Single Cells Using Droplet Microfluidics....Pages 71-94
Microfluidic Approaches to Fluorescence In Situ Hybridization (FISH) for Detecting RNA Targets in Single Cells....Pages 95-112
Microfluidic Multistage Integration for Analysis of Circulating Exosomes....Pages 113-139
Microfluidic Single-Cell Functional Proteomics....Pages 141-159
Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays....Pages 161-187
Single-Cell Phenotypic Screening in Inverse Metabolic Engineering....Pages 189-204
Microfluidic Paper-Based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers....Pages 205-218
Droplet Microfluidics for Screening of Surface-Marker and Secretory Protein Expression....Pages 219-233
Wire-guided Droplet Manipulation for Molecular Biology....Pages 235-252
Mechanical and Electrical Principles for Separation of Rare Cells....Pages 253-294
Microfluidics for High-Throughput Cellular Isolation and Analysis in Biomedicine....Pages 295-322
Microfluidics for Cell Culture....Pages 323-347
Microfluidic Chromatin Immunoprecipitation for Analysis of Epigenomic Regulations....Pages 349-363
Back Matter....Pages 365-376

✦ Subjects

Medical Microbiology; Biomedical Engineering; Biochemical Engineering