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Microfluidic Isolation of Leukocytes from Whole Blood for Phenotype and Gene Expression Analysis

✍ Scribed by Sethu, Palaniappan; Moldawer, Lyle L.; Mindrinos, Michael N.; Scumpia, Philip O.; Tannahill, Cynthia L.; Wilhelmy, Julie; Efron, Philip A.; Brownstein, Bernard H.; Tompkins, Ronald G.; Toner, Mehmet

Book ID
127317482
Publisher
American Chemical Society
Year
2006
Tongue
English
Weight
527 KB
Volume
78
Category
Article
ISSN
0003-2700

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## Abstract Analysis of gene expression is emerging as a valuable tool in drug discovery. Using a gene expression system involving high‐precision quantitative PCR, two robust in vitro models of inflammation in human whole blood were examined. In the first model, lipopolysaccharide (LPS) addition to