✦ LIBER ✦

Microfluidic devices for DNA sequencing: sample preparation and electrophoretic analysis

✍ Scribed by Brian M Paegel; Robert G Blazej; Richard A Mathies

Publisher: Elsevier Science
Year: 2003
Tongue: English
Weight: 418 KB
Volume: 14
Category: Article
ISSN: 0958-1669
DOI: 10.1016/s0958-1669(02)00004-6

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✦ Synopsis

Modern DNA sequencing 'factories' have revolutionized biology by completing the human genome sequence, but in the race to completion we are left with inefficient, cumbersome, and costly macroscale processes and supporting facilities. During the same period, microfabricated DNA sequencing, sample processing and analysis devices have advanced rapidly toward the goal of a 'sequencing lab-on-a-chip'. Integrated microfluidic processing dramatically reduces analysis time and reagent consumption, and eliminates costly and unreliable macroscale robotics and laboratory apparatus. A microfabricated device for high-throughput DNA sequencing that couples clone isolation, template amplification, Sanger extension, purification, and electrophoretic analysis in a single microfluidic circuit is now attainable.

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