Microflow Vessel Improving Reproducibility and Sensitivity of Electrochemical Measurements

✍ Scribed by Jan Krejci; Karel Lacina; Hana Vránová; Zuzana Grosmanová

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 497 KB
• Volume: 20
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200804364

✦ Synopsis

Abstract

A new microflow system was designed and developed for electrochemical measurements. The electrochemical electrodes prepared using thick film technology were used in this arrangement. Results of various measurements such as simple amperometric measurement on the example of H~2~O~2~ detection, measurement with glucose oxidase (GOx) biosensor, soluble enzyme activity measurement etc. carried out using this system are reported. It was observed that the sensitivity and reproducibility of the electrochemical measurements is improved significantly. The new device performance was proved on H~2~O~2~ detection, activity of GOx measurement and heavy metals detection (measured concentration range: H~2~O~2~ 10^−9^ to 10^−1^ M, glucose 10^−6^ to 10^−2^ M, activity of GOx 10^−1^ to 10^2^ IU, heavy metals (Cu, Pb) 10^−4^ to 10^−3^ M). The microflow insert greatly reduces the overall size of the electrolyte vessel and measurements with sample quantity as low as 2 mL can be accomplished.