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Microfabricated Polymer Devices for Automated Sample Delivery of Peptides for Analysis by Electrospray Ionization Tandem Mass Spectrometry

✍ Scribed by Chan, Jason H.; Timperman, Aaron T.; Qin, Dong; Aebersold, Ruedi

Book ID
127270642
Publisher
American Chemical Society
Year
1999
Tongue
English
Weight
152 KB
Volume
71
Category
Article
ISSN
0003-2700

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An on-line method has been developed for the derivatization and coupled liquid chromatography (LC)/electrospray ionization (ESI) MS analysis of peptides at the femtomol level. Peptides are reacted with N-succinimidyl-2(3-pyridyl)acetate (SPA) in buffered aqueous medium at pH 7 following loading on a