Microemulsion electrokinetic capillary chromatography of sulfated disaccharides derived from glycosaminoglycans

✍ Scribed by Orthodoxia Mastrogianni; Fotini Lamari; Alexandra Syrokou; Maria Militsopoulou; Anders Hjerpe; Nikos K. Karamanos

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 45 KB
• Volume: 22
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/1522-2683(200108)22:13<2743::aid-elps2743>3.0.co;2-k

✦ Synopsis

Microemulsion electrokinetic capillary chromatography of sulfated disaccharides derived from glycosaminoglycans

Microemulsion electrokinetic capillary chromatography (MEEKC) is a capillary electrophoresis technique in which neutral and ionized species can be resolved according to their partitioning into moving oil droplets present in the operating buffer. In this report, we present for the first time the application of MEEKC in the analysis of glycosaminoglycans. An efficient method for the separation of the variously sulfated D-disaccharides obtained following digestion of chondroitin and dermatan sulfates with chondro/ dermato lyases and derivatization with 2-aminoacridone is described. Nonsulfated, mono-, di-, and trisulfated D-disaccharides were completely separated using the microemulsion octane/butan-1-ol/Sodium dodecyl sulfate (SDS) in 10 mM borate buffer, pH 9.3, at 25 kV. Agreement of the obtained disaccharide composition with literature values showed that MEEKC can be used for the analysis of glycosaminoglycans.