Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels

✍ Scribed by Scott L. Nyberg; Steven M. Santilli; Russell A. Shatford; Frank B. Cerra; David R. Knighton; Wei -Shou Hu

• Book ID: 104646522
• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 314 KB
• Volume: 5
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00155491

✦ Synopsis

SLJMMARY

Mrcroelectrodcs have been used for the measurement of oxygen tension in various biological systems&Silver, 1987) Although not previously reported, microclcctrodes allow the direct measurement of oxygen tension profiles within collagen gels containing entrapped hcpatocytcs (collagen-hepatocyte gels). These oxygen tension profiles, along with hepatocyte oxygen consumption data, allowed the estimation of a diffusion coefficient for oxygen in collagen-hcpatocyte gels, %?& = 2.99 x 105 cm2/s.