Microdegradation procedure for radioactive peptide ergot alkaloids
✍ Scribed by G. Basmadjian; H. G. Floss
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 555 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0022-3549
No coin nor oath required. For personal study only.
✦ Synopsis
Radioactivity determinations were carried out in a Beckman LS 100 liquid scintillation spectrometer, using 10 ml. of a toluene scintillator solution (7 g. 2,5-diphenyloxazole and 0.3 g. dirnethyl-l,Cbis[2-(4methyI-5-phenyloxazolyl)]benzene/I000 ml. toluene) for compounds dissolved in organic solvents or 10 ml. of Bray's solution Abstract c1 A degradation procedure for **C-Iabeled peptide ergot alkaloids is described; it allows the determination of the specific activities of all the products obtained in the hydrolysis of these alkaloids from a sample of not more than 10 umoles.
(7) for aqueous samples.
counting the sample after the addition of a known amount of toluene-14C as internal standard. Colorimetric determinations were Keyphrases Ergot alkaloids, radioactive peptides-biosynthesis,
The counting efficiency for each sample was determined by redegradation, isotope distribution 0 Peptide ergot alkaloids, radiolabeled-degradation, distribution TLC-separation, analysis