Microcolumn supercritical fluid chromatography with on-line Fourier transform infrared detection

✍ Scribed by Mark W. Raynor; Anthony A. Clifford; Keith D. Bartle; Colin Reyner; Alan Williams; Bernard W. Cook

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 795 KB
• Volume: 1
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220010210

✦ Synopsis

The combination of capillary supercritical fluid chromatography (SFC) and Fourier transform infrared spectrometry (FTIR) is demonstrated using a low-volume (0.8 pL) flow cell capable of withstanding high pressures. The design of the interface is presented, and its effect on peak shape and detector sensitivity is discussed. In order to optimize the system for use with 50-pm i.d. capillary columns, makeup fluid is added to the column effluent before it passes through the flow cell to a flame ionization detector (FID). Loss in chromatographic resolution caused by the cell's dead volume is therefore minimized, which is clearly demonstrated by the analysis of two ultraviolet curing coatings using carbon dioxide as a mobile phase. This application highlights the analytical power of the system for separating and identifying reactive monomers, oligomers, and photoinitiators in low-to medium-molecular-weight polymer systems.