𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation

✍ Scribed by Yu Liang; Yongzheng Cong; Zhen Liang; Lihua Zhang; Yukui Zhang


Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
187 KB
Volume
30
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Improved protein separation by microchip
✍ Yongzheng Cong; Yu Liang; Lihua Zhang; Weibing Zhang; Yukui Zhang πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 English βš– 405 KB

## Abstract A stepwise gradient of electric field strength was proposed for microchip IEF‐based protein separation, by which after focusing at low voltages, IEF was performed by applying higher separation voltages step‐by‐step. A linear relationship between the focusing time and the inverse of the

Integration of capillary isoelectric foc
✍ Tingting Wang; Junfeng Ma; Guijie Zhu; Yichu Shan; Zhen Liang; Lihua Zhang; Yuku πŸ“‚ Article πŸ“… 2010 πŸ› John Wiley and Sons 🌐 English βš– 216 KB

## Abstract An integrated platform consisting of protein separation by CIEF with monolithic immobilized pH gradient (M‐IPG), on‐line digestion by trypsin‐based immobilized enzyme microreactor (trypsin‐IMER), and peptide separation by CZE was established. In such a platform, a tee unit was used not

Improved method for identification of pr
✍ Dr. Robert B. Mackin; Veronica A. Raker; Joachim Spiess πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 360 KB πŸ‘ 1 views

Recent advances in protein sequence analysis now permit the determination of partial N-terminal and internal primary structure from low picomole quantities of protein. The major remaining hurdles to sequence analysis of small amounts of protein are the identification, isolation, and handling of micr