Microchip-based capillary electrophoresis for determination of lactate dehydrogenase isoenzymes

✍ Scribed by Gui-Sheng Zhuang; Jing Liu; Chun-Ping Jia; Qing-Hui Jin; Jian-Long Zhao; Hui-Min Wang

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 807 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600506

✦ Synopsis

Abstract

This article describes a novel microchip‐based capillary electrophoresis and on‐column enzymatic reaction analysis protocol for lactate dehydrogenase (LDH) isoenzymes with a home‐made xenon lamp‐induced fluorescence detection system. A microchip integrated with a temperature‐control unit is designed and fabricated for low‐temperature electrophoretic separation of LDH isoenzymes, optimal enzyme reaction temperature control, and product detection. A four‐step operation and temperature control are employed for the determination of LDH activity by on‐chip monitoring of the amount of incubation product of NADH during the fixed incubation period and at a fixed temperature. Experiments on the determination of LDH standard sample and serum LDH isoenzymes from a healthy adult donor are carried out. The results are comparable with those obtained by conventional CE. Shorter analysis times and a more stable and lower background baseline can be achieved. The efficient separation of different LDH forms indicates the potential of microfluidic devices for isoenzyme assay.