Microchip-Based Assay Systems: Methods and Applications (Methods in Molecular Biology)

✍ Scribed by Pierre N. Floriano

• Publisher: Humana Press
• Year: 2007
• Tongue: English
• Leaves: 235
• Series: Methods in Molecular Biology
• Edition: 1
• Category: Library

✦ Synopsis

This volume explores recent progress in the microelectronics arena, the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world. While the area of microelectronics is faced with daunting challenges, it promises fascinating new advances that may translate into products such as sensors and devices that use nanometer-sized building blocks for real-world applications. The contributors to this volume are part of growing interdisciplinary efforts among pioneers in microelectronics, nanoscience, and health that will continue to create exciting new microchip-based applications across a variety of sectors. In the 16 chapters of this volume, leading scientists present technically precise microchip-based assays for a variety of applications. Highlights include: methods for microchip electrophoresis for DNA separation; fabrication of porous polymer monoliths in microfluidic chips for selective nucleic acid concentration and purification; rapid electrical lysis of bacterial cells in a microfluidic device; an on-chip bioassay that uses immobilized sensing bacteria in a three-dimensional microfluidic network; and impedimetric detection for DNA hybridization within microfluidic biochips. Molecular diagnostic and immunologic protocols include microchip-based enumeration of human white blood cells; microchip diagnosis of cervical cancer; protocols for DNA microchips toward molecular signatures in cervical cancer; a microchip based assay for Interleukin-6; and methods for microchip-based electrochemical enzyme immunoassays. Several chapters are devoted to topics in microarray technology, including: applications of functional protein microarrays; allergen microarrays for the diagnosis of specific IgE in a multiplex biochip-based immunoassay; protein-array based multiplexed cytokine assays; and lectin microarrays for glycoprotein assays.

✦ Table of Contents

Cover......Page 1
Frontmatter......Page 2
1 Microchip Electrophoresis for DNA Separation by Wire-Imprinted Microchannels on PMMA Substrates......Page 13
2 Fabrication of Porous Polymer Monoliths in Microfluidic Chips for Selective Nucleic Acid Concentration and Purification......Page 21
3 Rapid Electrical Lysis of Bacterial Cells in a Microfluidic Device......Page 34
4 On-Chip Bioassay Using Immobilized Sensing Bacteria in Three-Dimensional Microfluidic Network......Page 47
5 Microchip-Based Enumeration of Human White Blood Cells......Page 63
6 Microchips for the Diagnosis of Cervical Cancer......Page 75
7 DNA Microchips to Identify Molecular Signatures in Cervical Cancers......Page 97
8 Impedimetric Detection for DNA Hybridization Within Microfluidic Biochips......Page 112
9 Applications of Functional Protein Microarrays......Page 130
10 A Microchip-Based Assay for Interleukin-6......Page 140
11 Allergen Microarrays for the Diagnosis of Specific IgE Against Components of Cow’s Milk and Hen’s Egg in a Multiplex Biochip-Based Immunoassay......Page 154
12 Surface Plasmon Resonance Imaging on Polypyrrole Protein Chips......Page 167
13 Protein Array-Based Multiplexed Cytokine Assays......Page 184
14 Lectin Microarrays for Glycoprotein Analysis......Page 200
15 Interaction of HIV RNA With Peptides Detected by Acoustic Shear Wave Sensor Operated in an On-line Format......Page 211
16 Microchip-Based Electrochemical Enzyme Immunoassays......Page 221
Index......Page 231