## Abstract The detection of negative‐strand hepatitis C virus (HCV) RNA is a hallmark of replication. A highly sensitive and specific method is required to quantify the very low level of replication inherent to in vitro infection systems. Based on reverse transcription with a tagged primer in the
Microchip and capillary electrophoresis for quantitative analysis of hepatitis C virus based on RT-competitive PCR
✍ Scribed by Kung-Chia Young; Hsiang-Mei Lien; Chun-Che Lin; Ting-Tsung Chang; Gwo-Bin Lee; Shu-Hui Chen
- Book ID
- 117222841
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 211 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0039-9140
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A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr
## Abstract Accuracy for monitoring of the concentration of hepatitis C virus (HCV) RNA represents a major challenge throughout the management of patients with chronic hepatitis C. To investigate the genotype‐independent efficiency and the accuracy of two real‐time detection reverse transcription‐p