Microbioluminometry assay: Determination of erythromycin activity in plasma or serum

✍ Scribed by Peter E. Andreotti; Irwin S. Morse; Diana M. Hartmann

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 764 KB
• Volume: 78
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600781202

✦ Synopsis

A microbioluminometry assay (MBA) was developed for the quantitative analysis of erythromycin activity in human plasma or serum. The MBA method is adapted from turbidimetric methods and utilizes an enzyme-catalyzed bioluminescence reaction to quantitate the growth of Staphylococcus aureus in liquid culture medium. Statistical analysis of data obtained in method validatioin studies and in more than 500 assays of standard curve and control samples demonstrates consistent reproducibility and accuracy within the'oretical limits. The MBA was shown to be more sensitive than agar diffusion assays with a lower limit of sensitivity less than 20.0 ngimL and coefficients of variation less than 10%. Cumulative results of 178 as,says of spiked control plasma samples in the range of 0.1 4-2.1 8 WgirnL SI TOW 1 1.2% of individual determinations are > 215% the expected value, and 5.6% of individual determinations