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Microbial transformation of azacarbazoles X: Regioselective hydroxylation of 5,11-dimethyl-5H-indolo [2,3-b]quinoline, a novel DNA topoisomerase II inhibitor, byRhizopus arrhizus

✍ Scribed by Wanda Peczyńska-Czoch; Jarosław Osiadacz; Łukasz Kaczmarek; Tomasz Żal


Book ID
104633210
Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
387 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis


Microbial transformation of cytotoxic 5,1 l-dimethyl-5H-indolo [2,3-b]quinoline (a compound displaying antitumor activity and affecting the activity of calf thymus DNA topoisomerase 1I) was performed by the Rhizopus arrhizus strain and yielded a 9-hydroxy derivative. The metabolite obtained displayed a stronger cytotoxity against KB cells than the parenat compound (IDs0=0.001 !Ltmol/mL), and stimulated also the formafon of calf thymus topoisomerase II mediated pSP65 DNA cleavage in vitro at the concentration of 3 [aM. Being analogous to 9-hydroxyellipticine (wluch is an antitumor alkaloid), this novel indolo[2,3-b] quinoline derivative can be regarded as a novel potential antitumor agent.