Microbial synthesis of 3-deoxy-d-erythro-hex-2-ulosonic acid 6-phosphate

✍ Scribed by Bernd R. Knappmann; Mohamed A. El-Nawawy; Hans G. Schlegel; Maria-Regina Kula

• Book ID: 102990326
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 538 KB
• Volume: 242
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(93)80029-e

✦ Synopsis

A microbial route was explored for the synthesis of 3-deoxy-D-erythro-hex-2-ulosonic acid 6-phosphate (2-keto-3-deoxy-6-phosphogluconate, KDPG). Two strains of bacteria, Alcaligenes eutrophus H16 F34 (DSM 529) and Escherichia coli DF 71 (CGSC 4880), lacking in KDPG-aldolase activity were tested for excretion of KDPG. Using pyruvate and gluconate as carbon sources, Alcaligenes eutrophus H16 F34 accumulated and excreted 3-deoxy-D-erythro-hexulosonic acid 6-phosphate into the culture broth, while the E. coli strain, using pyruvate and glucuronate, failed. KDPG was isolated from the culture supernatant of Alcaligenes eutrophus H16 F34 in 78% yield and 5 g scale with respect to the consumed gluconate.