𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Microbial production of l-[15N]glutamic acid and its gas chromatography-mass spectrometry analysis

✍ Scribed by Z.E. Kahana; A. Lapidot

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
348 KB
Volume
132
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

L-[15N]Glutamic acid was prepared in high yields via a fermentative process. Brevibacterium lactofermentum, growing on a medium containing 97% enriched 15NH4Cl as a sole isotopic precursor, excreted mostly L-[15N]glutamic acid. The L-[15N]glutamic acid was purified and identified. Gas chromatography-mass spectrometry analysis was performed to demonstrate its usefulness in clinical studies.

πŸ“œ SIMILAR VOLUMES

Use of L-[15N] glutamic acid and homoglu
Use of L-[15N] glutamic acid and homoglutathione to determine both glutathione synthesis and concentration by gas chromatography-mass spectrometry (GCMS)
✍ Bernard Humbert; Patrick Nguyen; Christiane Obled; Christine Bobin; Anne Vaslin; πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 178 KB

A method for simultaneous measurement of both glutathione enrichment and concentration in a biological sample using gas chromatography mass spectrometry is described. The method is based on the preparation of N,S-ethoxycarbonylmethyl ester derivatives of glutathione, and the use of homoglutathione (

Analysis of domoic acid and related comp
Analysis of domoic acid and related compounds by mass spectrometry and gas chromatography/mass spectrometry as N-trifluoroacetyl-O-silyl derivatives
✍ Stephen Pleasance; Mei Xie; Yves LeBlanc; Michael A. Quilliam πŸ“‚ Article πŸ“… 1990 πŸ› John Wiley and Sons 🌐 English βš– 763 KB

A method is presented for the analysis of shellfish tissue for domoic acid, a neurotoxic amino acid responsible for cases of amnesic shellfish poisoning. Tissue extracts are first taken through a two-stage solid-phase extraction clean-up, using reversed-phase and strong cation exchange cartridges. A