Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine byPseudomonas putidaM10
✍ Scribed by Neil C. Bruce; Clare J. Wilmot; Keith N. Jordan; Anna E. Trebilcock; Lauren D. Gray Stephens; Christopher R. Lowe
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 539 KB
- Volume
- 154
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
A strain of Pseudomonas putida was isolated by selective enrichment with morphine that was capable of utilising morphine as a primary source of carbon and energy for growth. Experiments with whole cells showed that both morphine and codeine, but not thebaine, could be utilised. A novel NADP-dependent dehydrogenase, morphine dehydrogenase, was purified from crude cell extracts and was shown to be capable of oxidising morphine and codeine to morphinone and codeinone, respectively. This NADP-dependent morphine dehydrogenase was not observed in any other species of pseudomonads examined and was quite distinct from the beta-hydroxysteroid dehydrogenase found in Pseudomonas testosteroni, which had previously been shown to have activity against morphine.