Microbial conversion of oleic acid to 10-hydroxystearic acid

✍ Scribed by S. Koritala; C. T. Hou; C. W. Hesseltine; M. O. Bagby

• Book ID: 104655033
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 500 KB
• Volume: 32
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00184978

✦ Synopsis

Resting cell suspensions of seven Nocardia species catalyzed the production of 10-hydroxystearic acid from oleic acid. Nocardia choles- terolicum N R R L 5767 gave a good yield with optimum conditions at pH 6.5 and 40 ° C. Yields exceeding 90% can be obtained within 6 h with 0.1 g cells (dry weight) and 178 mg oleic acid in 10 ml of 0.05 M sodium phosphate buffer (pH 6.5). In addition, minor amounts of 10-ketostearic acid were formed as a by-product. The reaction proceeded via hydration of the double bond as shown by labeling experiments with deuterium oxide and 180-labeled water. The system was specific for fatty acids with cis unsaturation at the 9 positon.