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Microbead-based rolling circle amplification in a microchip for sensitive DNA detection

✍ Scribed by Sato, Kae; Tachihara, Atsuki; Renberg, Björn; Mawatari, Kazuma; Sato, Kiichi; Tanaka, Yuki; Jarvius, Jonas; Nilsson, Mats; Kitamori, Takehiko

Book ID
121688750
Publisher
Royal Society of Chemistry
Year
2010
Tongue
English
Weight
235 KB
Volume
10
Category
Article
ISSN
1473-0197

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CE combined with rolling circle amplific
CE combined with rolling circle amplification for sensitive DNA detection
✍ Ni Li; Jishan Li; Wenwan Zhong 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 405 KB

## Abstract Here we describe an assay which combines CE with rolling circle amplification (RCA) for sensitive DNA detection and quantification. RCA is an isothermal DNA replication technique that generates a long ssDNA with tandem repeats. It requires simpler temperature control in reaction and off