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Microassay for rapid measurement of 7-ethoxyresorufin-O-deethylase activity in intact fish hepatocytes

✍ Scribed by Anja Behrens; Kristin Schirmer; Niels C. Bols; Helmut Segner


Book ID
117313942
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
394 KB
Volume
46
Category
Article
ISSN
0141-1136

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An alternative 7-ethoxyresorufin o-deeth
✍ Alan V. Klotz; John J. Stegeman; Chris Walsh πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 686 KB

A procedure to directly measure the cytochrome P-450-dependent 7-ethoxyresorufin O-deethylase activity with a visible spectrophotometer is described and compared to the standard fluorometric method. The two assays yielded identical results with both beta-naphthoflavone-treated mammalian (rat) and fi