Microarrays: Preparation, Microfluidics, Detection Methods, and Biological Applications

✍ Scribed by Leming Shi, Roger G. Perkins, Weida Tong (auth.), Kilian Dill, Robin Hui Liu, Piotr Grodzinski (eds.)

• Publisher: Springer-Verlag New York
• Year: 2009
• Tongue: English
• Leaves: 355
• Series: Integrated Analytical Systems
• Edition: 1
• Category: Library

✦ Synopsis

The area of microarrays has evolved from genomics, and has been applied to proteomics and single cell studies as well. The applications of microarrays benefit diverse fields ranging from fundamental systems biology to practical diagnostic tests. This book provides a critical analysis of the applications of microarrays in several key areas of bioanalytical research including DNA studies, peptide arrays, protein arrays, combinatorial chemistry arrays, cell-based arrays, and glycoarrays. Further progress in microarrays is being spurred by the advances in micro- and nano-fabrication technologies, modern molecular biology, and data mining. This book is designed to be a useful source of fundamental and current information for microarray research practitioners and for those who are just entering this field.

✦ Table of Contents

Front Matter....Pages i-xv
The Current Status of DNA Microarrays....Pages 3-24
Electrochemical Detection on Microarrays....Pages 25-34
Fully Integrated Microfluidic Device for Direct Sample-to-Answer Genetic Analysis....Pages 37-65
Integrated Microfluidic Devices for Automated Microarray-Based Gene Expression and Genotyping Analysis....Pages 67-93
Intensity Concentration Relationships for Electrochemical Detection....Pages 97-117
Genotyping Arrays....Pages 121-138
Peptide-Based Microarray....Pages 139-167
Protein Microarrays for the Detection of Biothreats....Pages 169-190
Photo-Generation of Carbohydrate Microarrays....Pages 191-210
Expression Profiling Using Microfluidic Living Cell Arrays....Pages 211-226
New Approaches to the Synthesis of Addressable Microarray Molecular Libraries....Pages 227-246
eSensor®A Microarray Technology Based on Electrochemical Detection of Nucleic Acids and Its Application to Cystic Fibrosis Carrier Screening....Pages 247-260
Use of Redox Enzymes for the Electrochemical Detection of Sequence-Specific DNA and Immunochemical Entities....Pages 263-270
Biochip Platforms for DNA Diagnostics....Pages 271-297
MagArray Biochips for Protein and DNA Detection with Magnetic Nanotags: Design, Experiment, and Signal-to-Noise Ratio....Pages 299-314
Bar Coding Platforms for Nucleic Acid and Protein Detection....Pages 315-338
Electrochemical Nanoparticle-Based Sensors....Pages 339-353
Back Matter....Pages 355-356

✦ Subjects

Analytical Chemistry; Biotechnology; Biochemical Engineering; Electrochemistry; Industrial Chemistry/Chemical Engineering; Characterization and Evaluation of Materials