Microarray Technology and Its Applications

✍ Scribed by Ralph R. Martel, Matthew P. Rounseville, Ihab W. Botros, Bruce E. Seligmann (auth.), Uwe R. Müller Ph.D., Prof. Dan V. Nicolau (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2005
• Tongue: English
• Leaves: 387
• Series: Biological and Medical Physics, Biomedical Engineering
• Edition: 1
• Category: Library

✦ Synopsis

The genomics revolution would not have been possible without the 'parallelisation' offered by microarray technology. This technological - and commercial - success has been since emulated by other applications areas, with a tremendous amplification of innovation. This book describes the fundamentals and latest developments in microarray technology, as well as its future directions. It presents detailed overviews of the different techniques of fabricating microarrays, of the chemistries and preparative steps involved, of the different types of microarrays, and of the instrumentation and optical issues involved.

✦ Table of Contents

Array Formats....Pages 3-22
Biomolecules and Cells on Surfaces — Fundamental Concepts....Pages 23-44
Surfaces and Substrates....Pages 45-61
Reagent Jetting Based Deposition Technologies for Array Construction....Pages 63-72
Manufacturing of 2-D Arrays by Pin-printing Technologies....Pages 73-88
Nanoarrays....Pages 89-118
The Use of Microfluidic Techniques in Microarray Applications....Pages 119-145
Labels and Detection Methods....Pages 147-179
Marker-free Detection on Microarrays....Pages 181-207
Analysis of DNA Sequence Variation in the Microarray Format....Pages 211-227
High Sensitivity Expression Profiling....Pages 229-250
Applications of Matrix-CGH (Array-CGH) for Genomic Research and Clinical Diagnostics....Pages 251-263
Analysis of Gene Regulatory Circuits....Pages 265-276
Protein, Antibody and Small Molecule Microarrays....Pages 279-295
Photoaptamer Arrays for Proteomics Applications....Pages 297-308
Biological Membrane Microarrays....Pages 309-320
Use of Reporter Systems for Reverse Transfection Cell Arrays....Pages 323-333
Whole Cell Microarrays....Pages 335-343
Tissue Microarrays for Miniaturized High-Throughput Molecular Profiling of Tumors....Pages 345-360
Application of Microarray Technologies for Translational Genomics....Pages 361-374

✦ Subjects

Biomedicine general;Biophysics/Biomedical Physics;Biomedical Engineering;Nanotechnology;Physical Chemistry;Medicine/Public Health, general