Microarray-based genomic profiling reveals novel genomic aberrations in follicular lymphoma which associate with patient survival and gene expression status
✍ Scribed by Carsten Schwaenen; Andreas Viardot; Hilmar Berger; Thomas F. E. Barth; Stefan Bentink; Hartmut Döhner; Martina Enz; Alfred C. Feller; Martin-Leo Hansmann; Michael Hummel; Hans A. Kestler; Wolfram Klapper; Markus Kreuz; Dido Lenze; Markus Loeffler; Peter Möller; Hans-Konrad Müller-Hermelink; German Ott; Maciej Rosolowski; Andreas Rosenwald; Sandra Ruf; Reiner Siebert; Rainer Spang; Harald Stein; Lorenz Truemper; Peter Lichter; Martin Bentz; Swen Wessendorf
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 462 KB
- Volume
- 48
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Abstract
Follicular lymphoma (FL) is characterized by a large number of chromosomal aberrations. However, their exact genomic extension and involved target genes remain to be determined. For this purpose, we used array‐based intermediate‐high resolution genomic profiling in combination with Affymetrix™ gene expression analysis. Tumor specimens from 128 FL patients were analyzed for the presence of genomic aberrations and the results were correlated to clinical data sets and mRNA expression levels. In 114 (89%) of the 128 analyzed cases, a total of 688 genomic aberrations (384 gains/amplifications and 304 losses) were detected. Frequent genomic aberrations were: −1p36 (18%), +2p15 (24%), −3q (14%), −6q (25%), +7p (19%), +7q (23%), +8q (14%), −9p (16%), −11q (15%), +12q (20%), −13q (11%), −17p (16%), +18p (18%), and +18q (28%). Critical segments of these imbalances were delineated to genomic fragments with a minimum size down to 0.2 Mb. By comparison of these with mRNA gene expression data, putative candidate genes were identified. Moreover, we found that deletions affecting the tumor suppressor gene CDKN2A/B on 9p21 were detected in nontransformed FL grade I–II. For this aberration as well as for −6q25 and −6q26, an association with inferior survival was observed. © 2008 Wiley‐Liss, Inc.