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Microarray-Based Detection of Protein Binding and Functionality by Gold Nanoparticle Probes

โœ Scribed by Wang, Zhenxin; Lee, Jason; Cossins, Andrew R.; Brust, Mathias

Book ID
121280567
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
203 KB
Volume
77
Category
Article
ISSN
0003-2700

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We present a method based on surface-enhanced Raman spectroscopy and exploiting a protein-protein recognition process able to detect thrombin at subpicomolar concentrations. Gold nanoparticles (NPs) were capped with a bifunctional molecule capable of forming a covalent link with the aromatic residue