During the course of investigations of photosynthetic pigments in vitror-' as well as in viva, a simple micro-method was needed for qualitative and quantitative anlysis of chlorophyll components and minor components in chlorophyll preparations. Wilson and Nutting? developed a spectrophotometric method for the determination of chlorophylls as pheophytins at concentrations of 5 pg/ml. However, an amount of contaminant too small to cause a change in the shape of the visible absorption spectrum might go undetected if masked by a component present in greater concentration. Then, chromatographic separation of test materials made visual, densitometric and spectrophotometric detection of small amount of contaminants more sensitive. A number of investigations of analytical and separative methods for-plant pigments, including column9, paperg-", and thin-layer chromatography (TLC)7.9.W3, b ve been reported. It is only possible to analyse pigment amounts up to the order of 10B6 g using paper chromatography', and to detect qualitatively amounts of contaminant up to the order of low9 g using TLC3. However, it is very difficult to detect small amounts of contaminants with similar RF values to that of the main pigment using these techniques. Therefore, we have used micro-scale highperformance liquid chromatography (HPLC) to improve separability and sensitivity. In this paper, we report the preliminary results obtained with this method, which enables the micro-scale quantitative and qualitative analysis of photosynthetic pigments down to low9 g.