✦ LIBER ✦
Micro-isoelectric focusing of single pollen grains from Cucurbita pepo L
✍ Scribed by Gilles Gay; Catherine Kerhoas; Christian Dumas
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 259 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
A technique of isoelectric focusing of proteins from single pollen grains has been developed using ultrathin polyacrylamide gels. On a single gel, one hundred grains may be compared for total proteins or enzyme patterns. With this technique it was possible to reveal heterogeneity of pollen grains in a population at a genetic level and in relation to pollen viability.