Micro and macro GLC determination of ethambutol in biological fluids

✍ Scribed by Ching S. Lee; Leslie Z. Benet

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 434 KB
• Volume: 67
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600670407

✦ Synopsis

Previously available GLC methods for ethambutol may only be used to measure quantities of drugs much greater than those found in biological fluids such as plasma and dialysate. A previously published GLC method for plasma samples is extended to measure ethambutol in dialysate. A second GLC method, involving derivatization with bis(trimethylsilyl)trifluoroacetamide and subsequent quantitation using a flame-ionization detector, is described for urine samples. With a dual-column and dual-detector gas--liquid chromatograph, simultaneous micro (plasma and dialysate) and macro (urine) determinations of ethambutol are possible.