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Michaelis-Menten Kinetics for Determining Enzymatic Activity of Lysostaphin

✍ Scribed by V. I. Surovtsev; T. V. Fedorov; M. A. Borozdina

Book ID
111574493
Publisher
Springer
Year
2004
Tongue
English
Weight
61 KB
Volume
69
Category
Article
ISSN
0006-2979

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A new graphical method was developed to determine the kinetic parameters in the Michaelis-Menten-type equation. This method was then applied to studying the kinetics of lactose hydrolysis by Aspergillus niger pgalactosidase. In this study, the reaction temperature ranged between 8 and 60"C, and the