Micellar liquid chromatography of polycyclic aromatic hydrocarbons: Alkylpyridinium chloride as mobile phase modifier and selective fluorescence quencher
✍ Scribed by Chunfeng Mao; Kelly E. McGill; Sheryl A. Tucker
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 373 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
The dual role of alkylpyridinium chlorides, cetylpyridinium chloride (CPC) and dodecylpyridinium chloride (DDPC), as micellar mobile phase modifiers and selective fluorescence quenching agents of polycyclic aromatic hydrocarbons (PAHs), in micellar liquid chromatographic separation of PAHs is reported. The replacement of 0.037 M cetyltrimethylammonium chloride in the aqueous mobile phase with CPC/DDPC quencher greatly simplifies the observed fluorescence‐detected chromatograms, facilitating PAH identification. The resulting chromatograms are similar to those obtained from the conventional approach – pyridinium chloride quencher in an acetonitrile mobile phase. To quantify the quenching, the (F~0~ /F – 1) values from the Stern‐Volmer equation are calculated from the chromatograms and compared. The feasibility of using CPC or DDPC as a dual reagent under isocratic and gradient conditions is shown.