Micellar electrokinetic chromatography in coated capillaries: Determination of micelle-aqueous partition coefficients

✍ Scribed by Janini, George M. ;Muschik, Gary M. ;Issaq, Haleem J.

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 355 KB
• Volume: 18
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240180307

✦ Synopsis

Abstract

A micellar electrokinetic chromatography method is presented for the determination of the partition coefficient for the distribution of nonpolar and moderately polar solutes between the micelle and the aqueous phases in aqueous micellar solutions. In comparison with the literature the method is, theoretically and experimentally, the most straightforward for this application. An equation is derived for the determination of the partition coefficient in a coated fused silica capillary, with neglible electroosmotic flow, from simple measurements of the migration times of solutes and a marker for micelle migration. The advantages and limitations of this method are discussed. The method is tested by using sodium dodecylsulfate (SDS) surfactant and naphthalene, benzene, toluene, and phenol solutes. Micellar electrokinetic chromatography in coated columns isideally suited for the separation of hydrophobic solutes in aqueous samples.