Micellar electrokinetic chromatography – From theoretical concepts to real samples (Review)

Amino acid analysis by micellar electrok

Amino acid analysis by micellar electrokinetic chromatography with laser-induced fluorescence detection: Application to nanolitre-volume biological samples from Arabidopsis thaliana and Myzus persicae

✍ Xunlin Zhu; P. Nicholas Shaw; Jeremy Pritchard; John Newbury; Emma J. Hunt; Davi 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 356 KB

## Abstract Amino acids were derivatised with 4‐fluoro‐7‐nitrobenzo‐2,1,3‐oxadiazol (NBD‐F), separated by micellar electrokinetic chromatography (MEKC), and detected by argon‐ion (488 nm) laser‐induced fluorescence. The optimised MEKC background electrolyte conditions were: 40 mM sodium cholate, 5