Micellar Electrokinetic Capillary Chromatography of Proteins

✍ Scribed by M.A. Strege; A.L. Lagu

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 855 KB
• Volume: 210
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1214

✦ Synopsis

Separations of model proteins obtained under denaturing conditions in the presence of micellar concentrations of ionic surfactants displayed high resolution and efficiency using either bare silica or C18-derivatized silica capillaries. Superior migration time reproducibility was achieved through the use of the C18-derivatized capillaries (run-to-run migration time (% \mathrm{RSD}=0.2) ), relative to that obtained in bare silica capillaries (runto-run migration time (% R S D=2.2) ), in the absence of buffer replenishment. The effects of surfactant concentration and (\mathrm{pH}) upon the separation of a mixture of five model proteins of varying ionic and hydrophobic character were investigated, and the application of this technique to the analysis of a recombinant DNA-derived protein in fermentation broth was demonstrated.

(c) 1993 Academic Press, Inc.