Micellar electrokinetic capillary chromatography determination of zinc bacitracin and nystatin in animal feed

✍ Scribed by Rade Injac; Javor Kac; Ales Mlinaric; Katarina Karljikovic-Rajic

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 837 KB
• Volume: 29
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200500477

✦ Synopsis

Abstract

An MEKC procedure was developed for the separation of zinc bacitracin (Zn‐BC) and nystatin (NYS) in mixtures and in animal feedstuff. The running buffer was 15 mM borate/19 mM phosphate, pH 8.2, containing 20 mM SDS and 10% v/v methanol. Samples were run at 25°C, the applied voltage was 25 kV, and an additional pressure of 5 mbar was applied. Both analytes were detected by UV simultaneously at 215 nm, Zn‐BC alone at 192 and 254 nm, and NYS alone at 305 nm. The method was shown to be specific, accurate (recoveries were 100.0 ± 0.6% and 100.1 ± 0.6% for Zn‐BC and NYS, respectively), linear over the tested range (correlation coefficients 0.9991 and 0.9994), and precise (RSD below 1.3% for both analytes). The method was applied to determine Zn‐BC and NYS as additives in animal feed.