MICA allele typing of HLA-B27 positive japanese patients with seronegative spondylarthropathies and healthy individuals: Differential linkage disequilibrium with HLA-B27 subtypes
✍ Scribed by Naoyuki Tsuchiya; Michiko Shiota; Satoshi Moriyama; Atsuko Ogawa; Miki Komatsu-Wakui; Hiroshi Mitsui; Daniel E. Geraghty; Katsushi Tokunaga
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 669 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Objective:
To examine whether mica (major histocompatibility complex [mhc] class i-related chain gene a) confers additional susceptibility for seronegative spondylarthropathies in hla-b27 positive japanese individuals.
Methods:
A polymerase chain reaction-single-strand conformation polymorphism method was developed, and the mica alleles of 18 japanese patients with ankylosing spondylitis, 1 patient with reiter's syndrome, and 17 healthy hla-b27 positive japanese subjects were determined.
Results:
Among 26 individuals with hla-b*2704 (13 patients and 13 healthy subjects), all except 1 healthy individual were positive for mica010, whereas all 9 hla-b*2705 positive subjects (6 patients and 3 healthy subjects) possessed mica007. one healthy individual with hla-b*2711 also carried mica010.
Conclusion:
Strong linkage disequilibrium is present between hla-b*2704 and mica010, as well as between hla-b*2705 and mica007. although hla-b*2704 and b*2705 are highly homologous, each subtype participates in a different mhc haplotype. direct involvement of mica polymorphism in the pathogenesis seems to be unlikely; however, such information will provide a useful tool for elucidating the evolutional pathway of hla-b27 subtypes as well as the contribution of other genes within the mhc region in the pathogenesis of these diseases.