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Methyltrypsin-Catalyzed Peptide Coupling: Comparison of Alkyl Ester and Guanidinophenyl Ester Derivatives as Acyl Donor Component

✍ Scribed by Kunihiko Itoh; Haruo Sekizaki; Eiko Toyota; Kazutaka Tanizawa

Book ID: 102564916
Publisher: Elsevier Science
Year: 1997
Tongue: English
Weight: 234 KB
Volume: 25
Category: Article
ISSN: 0045-2068
DOI: 10.1006/bioo.1997.1076

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✦ Synopsis

Methyltrypsin-catalyzed peptide synthesis has been studied by using conventional alkyl ester and p-guanidinophenyl ester derivatives of Ͱ-amino acid as the acyl donor component. They were found to be coupled with Ͱ-amino acid derivatives (acyl acceptor component) to produce dipeptide. The behavior of methyltrypsin toward both the substrates has been studied.

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