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Methyltransferase-Directed Derivatization of 5-Hydroxymethylcytosine in DNA

✍ Scribed by Zita Liutkevičiūtė; Dr. Edita Kriukienė; Indrė Grigaitytė; Dr. Viktoras Masevičius; Prof. Saulius Klimašauskas

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
601 KB
Volume
123
Category
Article
ISSN
0044-8249

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✍ Keith E. Zucker; Arthur D. Riggs; Steven S. Smith 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 752 KB

## Abstract We have developed a facile procedure for the purification of DNA methyltransferase activity from human placenta. The procedure avoids the isolation of nuclei and the dialysis and chromatography of large volumes. A purification of 38,000‐fold from the whole cell extract has been achieved