Methyltransferase: An enzyme assay for microbial methylmercury formation in acidic soils and sediments
✍ Scribed by Steven D. Siciliano; David R. S. Lean
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 112 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0730-7268
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✦ Synopsis
Abstract
Methylmercury (MeHg) is a highly toxic form of mercury that bioaccumulates in aquatic food chains. However, methods to reliably identify sites of MeHg formation or to quantify MeHg production require the use of isotopic tracers, which limits their use. In this paper, a method is presented to quantify the methylation of mercury by a methyltransferase pathway. This methyltransferase pathway is one of the biochemical pathways responsible for biological mercury methylation. Protein is extracted from environmental samples, and mercury methyltransferase (HgMT) activity of soil extracts is calculated by assessing increases in methyltransferase activity induced by Hg addition. In enzyme extracts from pure cultures or soil samples, HgMT activity correlated with net MeHg production and Hg consumption, suggesting that HgMT activity can be used to estimate MeHg production in field samples. Over the course of a three‐month period in a freshwater wetland, HgMT activity correlated with net MeHg concentrations (r^2^ = 0.55; p < 0.057). Furthermore, HgMT activity predicted (r^2^ = 0.80; p < 0.01) gross MeHg formation in freshwater wetlands as well as in laboratory microcosms calculated using previously published rate constants. Our results show that a methyltransferase assay, in combination with demethylation estimates, accurately predicts MeHg formation under field and laboratory conditions. This assay does not require the use of mercury added to field samples to estimate activity but rather estimates the biological activity present in the soil by quantifying the amount of enzyme present in the soil. Such an assay is well suited for use in field surveillance programs assessing MeHg formation in a variety of environments.