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Methylglyoxal induces G:C to C:G and G:C to T:A transversions in the supF gene on a shuttle vector plasmid replicated in mammalian cells

✍ Scribed by Naoko Murata-Kamiya; Hiroyuki Kamiya; Hiroshi Kaji; Hiroshi Kasai


Book ID
117766558
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
157 KB
Volume
468
Category
Article
ISSN
1383-5718

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G:C β†’ T:A and G:C β†’ C:G transversions ar
✍ Akasaka, Susumu ;Takimoto, Koichi ;Yamamoto, Kazuo πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 682 KB

Escherichia coli K12 strain KS40 and plasmid pKY241 were designed for easy screening of supF mutations in plasmid pZ189. KS40 is a nalidixic acid-resistant (gyrA) derivative of MBM7070 (lacZ(am)CA7020). Using in vitro mutagenesis, an amber mutation was introduced into the cloned gyrA structural gene