Methylantimony compound formation in the medium of Scopulariopsis brevicaulis cultures: 13CD3-L-methionine as a source of the methyl group
✍ Scribed by Paul Andrewes; William R. Cullen; Jörg Feldmann; Iris Koch; Elena Polishchuk
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 72 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0268-2605
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✦ Synopsis
The filamentous fungus Scopulariopsis brevicaulis produces nonvolatile methylantimony compounds (found in the medium) when grown in antimony(III)-rich medium. To investigate the methyl source, 13 CD 3 -labelled L-methionine was added to the growth medium. After one month sodium borohydride reduction of media samples produced dimethylstibine and trimethylstibine. The methylstibines were separated on a packed GC column and obtained as gaseous fractions. Analysis of the methylstibines, in the gaseous fractions, by CGC-MS (ion-trap) established 13 CD 3 incorporation in both the trimethyl-and dimethyl-antimony compounds.