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Methylamine facilitates demonstration of specific uptake of diphtheria toxin by CHO cell and toxin-resistant CHO cell mutants

โœ Scribed by Eisuke Mekada; Kenji Kohno; Masahiro Ishiura; Tsuyoshi Uchida; Yoshio Okada

Book ID
115756238
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
408 KB
Volume
109
Category
Article
ISSN
0006-291X

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โœ Erik M. Schaefer; Joan M. Moehring; Thomas J. Moehring ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 977 KB

We studied the binding of '251-labeled diphtheria toxin (DTX) to receptors on monolayer cultures of Chinese hamster ovary cells (CHO-K1) and Vero cells. The number of DTX receptors detected on the cell surface was shown to be dependent on the cell density (number of cells per unit area). Cells at lo