Enzymatic analysis of guanine nucleotide
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Dietrich O.R. Keppler; Werner Kaiser
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Article
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1978
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Elsevier Science
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English
β 427 KB
GTP and GDP concentrations can be determined by a simple and specific spectrophotometric assay that uses commercially available enzymes. The conversion of GTP to GDP catalyzed by nucleosidediphosphate kinase in the presence of ADP enables the subsequent use of guanylate kinase which is coupled with