The latest volume in this series contains seven articles. D. S. MCLAI~EN, W. W. C. READ, Z. L. AWDEW ASH M. TCHLIAN consider the microdetermination of vitamin A and carotenoids in blood and tissue, describing in detail methods which require not more than o.5-ml or 0.5-g samples and wllich have been found suitable in their laboratory. In a timely contribution, D. S. JACKSON AND E. G. CLEARY deal with the determination of collagen and clastin, proteins which are being increasingly studied in numerous physiological and clinical investigations. The determination of @glucuronidases is the subject of W. H. FXSHMAN'S review which deals with the preparation and use of a number of substrates for the assay of the enzymes in various biological materials. C. A. ANuRRsRN gives an introduction to the electron-probe microanalyzer and its use in biochemistry, This instrument, which can be used to give an essentially complete chemical analysis of microstructures in histological tissue preparations 2% s&c is shown to have considerable importance in chemical pathology, dental research, and histo-and cyto-cllelnistry.
S. G. ANDERSON contributes a chapter on preparative zonal centrifugation and gives examples of the application of the technique to various types of biological material. C. H, WANG gives an account of radiorespirometry
and shows how the study of respiratory 'QCOs can be applied to the identification and estimation of metabolic pathways. Finally, J, D. WINEFORDNER, W. J. MCCARTHY AND I?. A. ST. JOHN deal with pllosplloril~~etry as an analytical approach in biochemistry and indicate how phosphorescence studies can be applied both quantitatively and qualitatively in biochemistry and pharmacy. Once again, as readers have come to expect from this series, a high standard is maintained. Experimental details are given in full, the applications of each technique are indicated and extensive lists of relevant references are supplied.
H. G. BRAY (Birmingham)
Awl. Clrirrr. Ada, 42 (rgG8) 346